| Home | Magazines | Editorial Board | Instruction | Subscribe Guide | Archive | Advertising | Template | Guestbook | Help |
| ZHAO Chenguang,XU Gang,JU Fen.Expression and influence of LINGO-1 in differentiation of spinal cord neural stem cells[J].Chinese Journal of Spine and Spinal Cord,2017,(9):832-838. |
| Expression and influence of LINGO-1 in differentiation of spinal cord neural stem cells |
| Received:May 30, 2017 Revised:September 06, 2017 |
| English Keywords:LINGO-1 Spinal cord derived neural stem cells Differentiation Rat |
| Fund:国家自然科学基金（编号：81100932）；中国博士后科研基金（编号：20100481518） |
|
| Hits: 3021 |
| Download times: 1338 |
| English Abstract: |
| 【Abstract】 Objectives: To investigate the expression and effect of LINGO-1 in differentiation of spinal cord derived neural stem cells(SpNSCs). Methods: SpNSCs were isolated from spinal cord in three 10-week-old Fisher 344 rats and cultured in vitro, stained with Nestin, and the expression of LINGO-1 on P3(on differentiation day 0, 1, 3, 5) was observed. SpNSCs were transfected with LINGO-1 shRNA lentiviral vector and Scramble-shRNA lentiviral vector to investigate the infection efficiency and RNA interference effect. SpNSCs of P4 were divided into RNAi group and control group. The RNAi group was transfected with LINGO-1 shRNA lentiviral vector and control group was transfected with Scramble-shRNA lentiviral vector. The proportions of neurons and astrocyte were measured by immunofluorescence histochemistry. Results: SpNSCs were Nestin positive. The expression of LINGO-1 IOD was 514±45, 715±68, 1017±92, 654±61 on the 0th, 1st, 3rd, 5th differentiation day respectively. The expression of LINGO-1 at the very 1st differentiation day increased and was 1.39 times of expression on the day 0, 1.98 times on the 3rd day, and then decreased to 1.36 times on the 5th day, the differences of all expressions on the 4 days were statistically significant(P<0.05). The rate of LINGO-1 shRNA lentivirus infection was higher than 90%, which reduced the protein expression of LINGO-1 to 26% expression of SpNSCs with Scramble-shRNA lentiviral vector infection. The proportion of neurons in RNAi group was 3.85 times of that of the control group, and the differentiation of astrocytes was 0.65 times of the control group, the proportions of neurons and astrocytes in two groups were significantly different(P<0.05). Conclusions: The expression of LINGO-1 increases in the early days of differentiation. Inhibition of LINGO-1 gene expression in spinal cord derived neural stem cell, can promote differentiation into neurons and reduce into astrocyte. |
| View Full Text View/Add Comment Download reader |
| Close |
|
|
|
|
|