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| GAO Rui,YUAN Wen,WANG Xinwei.The effect of olfactory ensheathing cells on neural cell-like differentiation of bone mesenchymal stem cells in vitro co-culture system[J].Chinese Journal of Spine and Spinal Cord,2014,(9):834-838. |
| The effect of olfactory ensheathing cells on neural cell-like differentiation of bone mesenchymal stem cells in vitro co-culture system |
| Received:May 19, 2014 Revised:July 28, 2014 |
| English Keywords:Bone mesenchymal stem cell Olfactory ensheathing cell Co-culture Differentiation |
| Fund:上海市自然科学基金青年项目（编号：12ZR1454500） |
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| English Abstract: |
| 【Abstract】 Objectives: To investigate the differentiation of bone mesenchymal stem cell(BMSC) induced by co-culturing with olfactory ensheathing cell(OEC). Methods: BMSC and OEC were isolated and cultured from 20 5-6-week-old 100g male rats. BMSC was isolated from rat femur, and OEC was isolated from rat olfactory epithelium. The co-culture system used was 6-well 0.4m Transwell co-culture system. The third generation cells were co-cultured within this system at a density of 2×104/cm2 and 3×104/cm2 for OEC and BMSC respectively. BMSC was processed for immunofluorescence and flow cytometry at 7d and 14d for the specific marker Nestin, neuronal class Ⅲ β-tubulin(Tuj-1) and glial fibrillary acidic protein(GFAP). Results: Immunocytochemistry showed BMSC could express Nestin, Tuj-1 and GFAP after 7d and 14d co-culture. Flow cytometry revealed that the positive rate of Nestin, Tuj-1 and GFAP was 46.3%, 76.5% and 23.2% after 7d co-culture; and the positive rate of Nestin, Tuj-1 and GFAP was 27.7%, 89.1% and 50% after 14d co-culture. Conclusions: BMSC can efficiently differentiate into neural-like cells when coculturing with OEC. |
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