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| CHEN Rongtao,SHOU Kangquan,FU Naxin.Experimental study of ozone injection for treating intervertebral disc degeneration with prolapsed type[J].Chinese Journal of Spine and Spinal Cord,2014,(9):815-821. |
| Experimental study of ozone injection for treating intervertebral disc degeneration with prolapsed type |
| Received:April 08, 2014 Revised:July 22, 2014 |
| English Keywords:Medical ozone Intervertebral disc degeneration Autoimmunity Rabbit |
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| English Abstract: |
| 【Abstract】 Objectives: To observe the influence of ozone injection on the abnormal autoimmunity of intervertebral disc degeneration(IDD) model(L6/7) in animal. Methods: An IDD model was established on rabbits. Then medical ozone was used for treatment while medical purified oxygen was used as the control. The immunoglobulin G and immunoglobulin M in serum were obtained at two weeks after the operation and examined by turbidimetry. The presence and distribution of antigen-antibody complexes in nucleus pulposus were examined by immunohistochemistry. And the levels of cytokine TNF-α, IL-1, IL-6 were detected by enzyme linked immunosorbent assay, and the BCA method was also used to analyze the activity of PLA2 in the nerve root. Results: After the operation, there were abnormal activities in the groups of model, ozone and oxygen, which had the scores of free walk pain showed as 2.81±0.13, 2.69±0.11 and 2.64±0.31 respectively, and improved when the medical ozone was applied. Compared with the levels of the immunoglobulin G and M as (8.98±0.77g/L, 2.40±0.25g/L) and (8.52±0.63g/L， 2.91±0.31g/L) on model group and medical oxygen group, the medical ozone group showed lower levels(6.05±0.49g/L, 1.98±0.28g/L, P<0.05); compared with the model group and medical oxygen group as (256±39pg/ml，404±19pg/ml, 231±41.3pg/ml） and (244±45pg/ml， 410±15pg/ml, 249±21.67pg/ml)(P<0.05) for the leves of cytokine TNF-α, IL-1, IL-6 in the serum, the medical ozone group also showed lower values(98±11pg/L，247±21pg/L， 149±23.4pg/L); compared with the model group and medical oxygen group for the expression of antigen-antibody complexes in the nucleus pulposus(27.49±12.84) and (29.55±10.38), the value of medical ozone group was lower(7.40±3.56)(P<0.05);furthermore, the level of PLA2 in the lumbar nerve root of medical ozone group(0.0189±0.0072μmol/min·L) was also lower than the model group and medical oxygen group as (0.1685±0.0103μmol/(min·L) and (0.1994±0.080μmol/min·L)(P<0.01). Conclusions: Medical ozone injection can an inhibit the abnormal autoimmunity of LDH model to an extent, which can be a main mechanism of therapy for the early stage of IDD. |
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