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| JIANG Gangqiang,RUAN Dike,ZHOU Mingyue.Comparison of the biological effects of two kinds of homologous mesenchymal stem cells on degenerative nucleus pulposus cells under the condition of non-contact co-culture[J].Chinese Journal of Spine and Spinal Cord,2014,(3):251-256. |
| Comparison of the biological effects of two kinds of homologous mesenchymal stem cells on degenerative nucleus pulposus cells under the condition of non-contact co-culture |
| Received:November 20, 2013 Revised:February 07, 2014 |
| English Keywords:Non-contact co-culture Homologous mesenchymal stem cells Degenerative nucleus pulposus cells Ccomparative study |
| Fund:基金支持项目国家科学自然基金（编号：81250019） |
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| English Abstract: |
| 【Abstract】 Objectives: To compare the biological effects of degenerative nucleus pulposus cells when co-culturing two kinds of people′s mesenchymal stem cells under a non-contact system. Methods: Bone marrow mesenchymal stem cells(BMSCs), adipose mesenchymal stem cells(ADSCs) and nucleus pulposus cells(NPCs) were obtained from the same patient, then the two kinds of stem cells were co-cultured respectively with degenerative nucleus pulposus cells in Transwell six-well plates under the non-contact condition, NPCs co-cultivation with ADSCs as group A, and NPCs co-cultivation with BMSCs as group B, nucleus pulposus cells cultured alone as control. One week later, the total RNA of 3 groups were extracted, then the Real-Time PCR technology was used to detect the relative gene expression of type Ⅱ collagen, proteoglycan and SOX-9. Results: The relative gene expression of type Ⅱ collagen protein, proteoglycan and SOX-9 in control group was 1.03±0.28, 1.21±0.40 and 0.94±0.34 respectively, in group A was 3.49±0.55, 3.88±2.11 and 2.41±0.91 respectively, while 7.60±1.89, 6.26±2.96 and 4.55±1.88 respectively in group B, The Real-Time PCR results showed that compared with the control group, the nucleus pulposus cells of group A and B presented a significant increase of type Ⅱ collagen protein, proteoglycan and SOX-9 gene expression(P<0.05), which also showed significant difference between groups A and B(P<0.05). Conclusions: Bone marrow mesenchymal stem cells and adipose mesenchymal stem cells can both stimulate and activate the degenerative nucleus pulposus cells under the condition of noncontact co-culture; with stronger effect on the former, which may act as a better option for biological treatment for degenerative disc diseases. |
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