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| YU Huangfei,MA Jingjing,FANG Ning.Effects of human amnion-derived mesenchymal stem cells transplantation on the expression of NGF mRNA, BDNF mRNA, Nogo-A mRNA in rats after spinal cord injury[J].Chinese Journal of Spine and Spinal Cord,2013,(10):924-929. |
| Effects of human amnion-derived mesenchymal stem cells transplantation on the expression of NGF mRNA, BDNF mRNA, Nogo-A mRNA in rats after spinal cord injury |
| Received:December 10, 2012 Revised:April 21, 2013 |
| English Keywords:Spinal cord injury Transplantation Human amnion-derived mesenchymal stem cell Neurotrophic factor Rats |
| Fund:贵州省科技厅联合基金资助项目[编号：黔科合J字LKZ (2010)19号] |
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| English Abstract: |
| 【Abstract】 Objectives: To observe the effects of human amniotic mesenchymal stem cells(hAMSCs) transplantation on the expression of nerve growth factor(NGF), brain-derived neurotrophic factor(BDNF) and axonal growth inhibitor(Nogo-A) mRNA in rats after spinal cord injury. Methods: Twenty-four adult female SD rats were randomly divided into transplantation group and control group. Transected spinal cord injury(SCI) model in rats was established. Isolation, culture and identification of hAMSCs in vitro were performed, and 10μg/ml DAPI was used to label cultured hAMSCs. About 3×106 hAMSCs absorbed by a microsyringe were transplanted into the head and tail end lesions immediately after establishing of animal model, the control group was injected by equal volume of PBS. The survival hAMSCs in the injured spinal cord were observed by fluorescence microscope at 3, 7 days post-operation. Meanwhile, the expression of NGF mRNA, BDNF mRNA and Nogo-A mRNA in the injured spinal cord was detected by RT-PCR. Results: The grafting hAMSCs were observed in the host spinal cord tissue at 3d and 7d post operation. RT-PCR results showed the expression of NGF mRNA in transplantation group was significantly higher than that in control group(P＜0.05, P＜0.01) regardless of timepoint, and the expression of Nogo-A mRNA was lower than that in the control group(P＜0.05). However, compared with the control group, the expression of BDNF mRNA at 3d after operation showed no significant difference(P＞0.05), but at 7d after operation, the expression of BDNF mRNA in transplantation group was remarkably higher than that of control group(P＜0.05). Conclusions: The transplantation of hAMSCs can regulate the expression of neurotrophic factors in injured spinal cord tissue, which may contribute to the improvement of neurological function of rats after SCI. |
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