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| WANG Kun,WANG Feng,WU Xiaotao.he expression, distribution and significance of stromal cell-derived factor-1 in the normal and degenerative intervertebral disc[J].Chinese Journal of Spine and Spinal Cord,2013,(10):915-923. |
| he expression, distribution and significance of stromal cell-derived factor-1 in the normal and degenerative intervertebral disc |
| Received:January 28, 2013 Revised:August 21, 2013 |
| English Keywords:Stromal cell-derived factor-1 Intervertebral disc degeneration Normal intervertebral disc |
| Fund:国家自然科学基金（项目编号：81071493、31070876） |
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| English Abstract: |
| 【Abstract】 Objectives: To compare the expressions of stromal cell-derived factor-1(SDF-1) in different areas of normal and degenerative intervertebral disc, and to explore the expression pattern of SDF-1 in five adjacent parts of the intervertebral disc, which was the inner nucleus pulposus(INP), transitional nucleus pulposus(TNP), inner annulus fibrous(IAF), outer annulus fibrous(OAF) and cartilage endplate(CEP). Methods: 20 patients with lumbar intervertebral disc herniation were collected as the degeneration group(the degenerated discs were all located in L4/5, confirmed by surgical pathology). 7 patients with acute bursting fracture and 3 patients with scoliosis were included as the control group. Intervertebral disc was divided into five areas, which included INP, TNP, IAF, OAF, CEP. HE staining was used to detect the structure of the intervertebral disc in both groups; immunohistochemistry, Western blot and rt-PCR were used to detect the expression and distribution of SDF-1 in the five adjacent areas between two groups. Finally, the relationship between the results of immunohistochemistry of SDF-1, donor age and Thompson classification of the intervertebral disc in both groups were analyzed. Results: In control group, the immunohistochemistry IOD value of SDF-1 in the above-mentioned five areas was 420.87±89.93, 407.80±100.76, 380.02±85.05, 342.89±63.76, 344.06±88.97 respectively; Gray value of Western-blot was 0.08±0.03, 0.08±0.05, 0.07±0.04, 0.05±0.03, 0.05±0.02 respectively; Gray value of rt-PCR was 0.22±0.06, 0.22±0.05, 0.20±0.04, 0.20±0.04, 0.20±0.04 respectively. As for the degeneration group, the OD value of SDF-1 in those five areas was 7355.13±2271.74, 5959.58±2436.23, 5397.49±3044.80, 1605.44±825.31, 361.91±104.22 respectively; Gray value of Western-blot was 0.55±0.29, 0.52±0.28, 0.42±0.18, 0.21±0.12, 0.06±0.04 respectively; Gray value of rt-PCR was 0.75±0.30, 0.71±0.26, 0.55±0.22, 0.36±0.17, 0.22±0.13 respectively. The expression of SDF-1 in the five areas in the degeneration group was higher than the control group(P<0.05). Besides, the expression of SDF-1 in the five areas presented as a gradient manner, with the highest in the inner annulus fibrosus and the lowest in the cartilage endplate, which showed statistically significant difference(P<0.05). However, the central nucleus pulposus and border area nucleus pulposus had no difference(P>0.05). The expressions of SDF-1 in five areas had a positive correlation with the donor age and the Thompson classification. Conclusions: SDF-1 is expressed widely in the degenerative and normal intervertebral disc, and the distribution of SDF-1 in degenerated intervertebral disc at adjacent areas was in a gradient manner. |
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