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| LIN Bin,HE Yongzhi,TANG Kai.Effect of resveratrol on the expression of TNF-α and IL-1 in dorsal root ganglion neuron after auto-transplantation of nucleus pulposus in rat model[J].Chinese Journal of Spine and Spinal Cord,2013,(4):364-369. |
| Effect of resveratrol on the expression of TNF-α and IL-1 in dorsal root ganglion neuron after auto-transplantation of nucleus pulposus in rat model |
| Received:May 30, 2012 Revised:December 14, 2012 |
| English Keywords:Resveratrol TNF-α IL-1 Autologous nucleus pulposus Dorsal root ganglion |
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| 【Abstract】 Objectives: To observe the effect of resveratrol on the expression of TNF-α and IL-1 in dorsal root ganglion(DRG) neuron after auto-transplantation of nuclear pulposus in rat model. Methods: Forty-eight Sprague-Dawley rats were divided randomly into 4 groups: fat+normal saline group(group A), nucleus pulposus+normal saline group(group B), nucleus pulposus+dexamethasone(group C) and nucleus pulposus+resveratrol group(group D), with 12 rats in each group. All rats underwent laminectomy, which exposed the dorsal nerve root of left L4 and L5, and then in group B, C and D, autologous nucleus pulposus was transplanted around the nerve root, while in group A, autologous adipose tissue was transplanted instead. Sevens days after operation, six rats in each group were killed randomly and the left dorsal nerve roots were obtained, while the other rats in group D, resveratrol was given by intraperitoneal injection once a day and lasted for 5 days. In group C dexamethason was given, and in group A and B, saline was given in the same way. Then, at the 14th day, dorsal nerve roots of left L4 and L5 obtained from the other rats experienced HE staining, immunohistochemical staining(IHC) for TNF-α and IL-1, the TNF-α positive cells and the IL-1 apoptosis cells were measured under light microscope. The experimental data were analyzed by SPSS 17.0 statistical software. Results: There was no significant inflammatory reaction in group A. At 7 days, in group B, C, D, the arrangement between cells in DRG were loose and presented hyperemia and edema, ganglion cells Nissl bodies disappeared, vacuolar degeneration, nucleus disappearance, inflammatory cells infiltration, capillary dilatation, vacuolar degeneration in some myelin were evidenced. At 14 days, the inflammatory reaction was still evidenced in group B, while the pathological changes in the DRG were lighter in group C and D compared with group B, which included fewer disappearance of nissl bodies, just fewer vacuoles degeneration in nissl bodies and fewer inflammatory cell infiltration, and also the myelin vacuolar degeneration was lighter. The expressions of TNF- α and IL-1 in group B, C and D were apparently higher than group A at 7 and 14 days(P<0.01). The expressions of TNF-α and IL-1 in group C and D were apparently lower compared with group B(P<0.01) at 14 days, but there was no difference between group C and D(P>0.05). Conclusions: Autologous nucleus pulposus can induce inflammatory reaction in the DRGs. Resveratrol can alleviate the reaction through reducing the expression of TNF-α and IL-1 in DRGs. |
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