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| LIU Hangtao,LIN Guoye,LI Pingsheng.The influence of osteogenic protein-1 on histologic changes of rabbit lumbar disc induced by aspirating the nucleus pulposus[J].Chinese Journal of Spine and Spinal Cord,2013,(1):54-60. |
| The influence of osteogenic protein-1 on histologic changes of rabbit lumbar disc induced by aspirating the nucleus pulposus |
| Received:July 20, 2012 Revised:October 31, 2012 |
| English Keywords:Osteogenic protein-1 Annulus fibrosus Ucleus pulposus Aspiration of nucleus pulposus Rabbit |
| Fund:莆田市科技计划项目（编号：2010S09-2） |
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| 【Abstract】 Objectives: To observe the influence of osteogenic protein-1(OP-1) on disc histologic changes induced by aspirating the nucleus pulposus. Methods: All the 32 Japanese rabbits′ posterior annulus fibrosus of L1/2 and L3/4 disc were stabbed by 21-gauge hypodermic needle, then some nucleus pulposus tissues were aspirated out. 25μl OP-1 was injected into L3/4 disc by using micro-injection syringe as the experimental group(OP-1 group). 25μl normal saline was taken injected into L1/2 as a blank control disc(Saline group). L2/3 was taken as a normal control disc(normal group). At 2, 4, 8 and 12 weeks after operation, each 8 rabbits were executed and the disc tissues of L1/2, L2/3, L3/4 were harvested and sliced rapidly, stained by Masson for observation. Results: In the normal intervertebral disc, nucleus pulposus tissue collagen was sparse and lined orderly. The cells were distributed as island. Anulus fibrosus collagen fibers were lined regularly, without distortion and delamination. The intervertebral disc showed no significant pathological change at 2, 4, 8 and 12 weeks after operation. In saline group at 2 weeks after operation, excalation of the nucleus pulposus, collagen disarrangement and a large inhomogeneous amorphous particles could be observed. At 4 weeks after operation, the nucleus pulposus tissue formed cracks. More cartilage cells appeared. Collagen fibers were seriously distorted. At 8 weeks after operation, nucleus pulposus tissue had wide fissure. Collagen was arranged extremely disorder. A few cells appeared just between cartilage-like cells and fibroblasts. Layered fissure was formed. Collagen fibers were extremely distorted, with part of fracture evidenced. Gelatinous nucleus pulposus of disc showed significant fibrosis at 12 weeks after operation. Fibroblasts increased in nucleus pulposus region, and the number of cartilage-like cells decreased. The annulus fibrosus appeared huge layered fissure, with part of fracture evidenced. In OP-1 group at 2 weeks after operation, the nucleus pulposus collagen arrangement was ordered. There were still more nucleus pulposus cells. The shape of anulus fibrosus was close to normal. At 4 weeks after operation, the nucleus pulposus tissue formed little cracks, and collagen disarranged. Nucleus pulposus cells decreased, with cartilage-like cells appeared. The inner annulus collagen fibers were distorted slightly. At 8 weeks after operation, nucleus pulposus tissue had more cracks and distortion of collagen. Cartilage-like cells increased. The inner annulus collagen fibers were distorted significantly. At 12 weeks after operation there were still morecartilage-like cells. A few cells appeared just between cartilage-like cells and fibroblasts. The whole layer of the annulus fibrosus collage fibers were distorted and disorganized, but the outer annulus were intact without huge fissure formation. Conclusions: OP-1 can retard disc degeneration of rabbit induced by puncturing lateral annulus fibrosus and aspirating the nucleus pulposus. |
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