| Home | Magazines | Editorial Board | Instruction | Subscribe Guide | Archive | Advertising | Template | Guestbook | Help |
| MA Xinlong,YANG Zhao,SUN Xiaolei.Effects of cyclic distraction stress on proliferation of human nucleus pulposus cells[J].Chinese Journal of Spine and Spinal Cord,2012,(7):645-650. |
| Effects of cyclic distraction stress on proliferation of human nucleus pulposus cells |
| Received:July 20, 2011 Revised:February 03, 2012 |
| English Keywords:Cyclic distraction stress Human nucleus pulposus Proliferation Cell cycle |
| Fund:国家自然科学基金青年基金项目（项目批准号：308011680）；天津市应用基础及前沿技术研究计划（项目编号：09JCZDJC19600）；天津市卫生局科研基金攻关项目（10KG111） |
|
| Hits: 2510 |
| Download times: 2473 |
| English Abstract: |
| 【Abstract】 Objectives: To investigate the effects of cyclic distraction stress on the proliferation of human nucleus pulposus cells(HNPCs). Methods: HNPCs were cultured in vitro and identified via the toluidine blue, safranin O and immunocytochemical stain of collagenⅡ. P3 HNPCs were inoculated on elasticity polymer. After HNPCs were attached, BioDynamic test instrument was used to exert different distraction strain(0, 5%, 10%, and 15%) on HNPCs for 3 hours; after that, HNPCs were cultured for 24 hours, and the flow cytometry was used to examine the cell cycle and calculate the S phase fraction and proliferative index. Results: The cell adhesion and growth were well under different strain for 3h; S phase fraction in 0, 5%, 10% and 15%strain groups was 1.86±1.22, 2.07±0.09, 7.56±1.40 and 7.55±3.25 respectively. Proliferative index in these groups was 3.47±2.52, 7.88±2.45, 16.05±1.51 and 14.7±0.61 respectively. Compared with 0 strain group, proliferative index in 5% strain group increased significantly(P<0.05). Compared with 0 and 5% strain group, S phase fraction and proliferative index in 10% and 15% strain groups increased significantly(P<0.05); but S phase fraction and proliferative index showed no significant difference between 10% and 15% strain groups(P＞0.05). Conclusions: The proliferation of HNPCs increases under some stress. |
| View Full Text View/Add Comment Download reader |
| Close |
|
|
|
|
|