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| WEI Kaibin,ZHANG Wenzheng,LIU Hong.Cryopreserved olfactory ensheathing cells transplantation for the treatment of spinal cord injury, an experimental study[J].Chinese Journal of Spine and Spinal Cord,2012,(4):357-361. |
| Cryopreserved olfactory ensheathing cells transplantation for the treatment of spinal cord injury, an experimental study |
| Received:June 19, 2011 Revised:November 30, 2011 |
| English Keywords:Cryopreservation Olfactory ensheathing cells Nerve fiber Spinal cord injury Outcome |
| Fund:基金项目：山东省医药卫生科技发展计划项目（2009HW091） |
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| English Abstract: |
| 【Abstract】 Objectives: To observe the effect of cryopreserved olfactory ensheathing cells(OECs) transplantation in situ on spinal cord injury (SCI) and the effect of hypothermia on OECs activity. Methods： 56 Wistar rats undergoing T10 spinal cord hemisection were divided into 4 groups: fresh OECs group(A), cryopreserved OECs group(B), D/F12 medium group(C) and control group(D). In group B, the OECs in logarithmic phase were cryopreserved for 3 months, after resuscitate, they (labeled with hoechst) were transplanted into the injured region, while fresh OECs were transplanted in group A. At day1 and 1, 2, 3, 4, 6, 8, 10 weeks after transplantation, combined behavioral score(CBS) was used to evaluate the locomotion. The tissues were sectioned to observe the survival and migration of OECs, the nerve fiber regeneration and NGFRp75 immunohistochemistry staining at 5 and 10 weeks after transplantion. Results: At day1, 2weeks, 10 weeks after transplantation, the combined behavioral score (CBS) for group A was 85.78±1.07, 58.80±5.00, 6.52±2.37 respectively; 86.12±1.29, 60.06±6.51, 8.15±2.26 for group B; 86.4±1.03, 66.28±7.00, 30.65±5.60 for group C; 86.75±1.37, 69.85±6.61, 34.13±5.38 for group D. Group A and B had more improvement than group C and D at each timepoint after transplantation for 2 weeks (P<0.05), while no significant difference was noted between group A and B as well as group C and D (P>0.05).At 5 weeks after transplantation, axons were noted to grow into the injury site in group A and B, and some nerve fiber reached the distal end of injury site at 10 weeks after transplantation, no nerve fiber was noted in the injury site in group C and D. Both at 5 and 10 weeks, under NGFRp75 immunohistochemicl staining, the OECs could be observed around the injury area in group A and B, while no similar findings was noted in group C and D. OECs were detected in the spinal cord after 5 weeks of transplantation in group A and B, and their migration was evidenced. Conclusions: As for the treatment outcome for SCI, no difference exists between the fresh OECs and the cryopreserved OECs transplantation. |
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