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| BAI Jinzhu,HONG Yi,GUAN Hua.Effect of oscillating electrical field on motor function recovery and axon regeneration in rat with spinal cord injury[J].Chinese Journal of Spine and Spinal Cord,2012,(4):352-356. |
| Effect of oscillating electrical field on motor function recovery and axon regeneration in rat with spinal cord injury |
| Received:November 15, 2011 Revised:December 29, 2011 |
| English Keywords:Spinal cord injury Oscillating electric field Rat Motor function recovery |
| Fund:基金项目：国家自然科学基金青年基金（30801222）,北京市科技新星计划（2007B070） |
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| English Abstract: |
| 【Abstract】 Objectives： To explore the influence of oscillation field on motor function recovery and axon regeneration in rat with spinal cord injury. Methods： Ninety SD rats with spinal cord injury induced by Allen method was randomly divided into experimental group and control group. The stimulating electrode were added in both groups. The experimental group received oscillation electric field as intervention, while the control group experienced the implanted stimulator alone rather than the oscillation electric field. The electric field intensity was 600μV/mm. Every 15 min, the polarity alternated. The power supply mode was inductive system, and the rats received the stimulation in conscious state. At 2w, 6w, 12w after the experiment, BBB scores, motor evoked potentials (the MEP latency and amplitude difference), HE dyeing histology, neurofilament protein (NF200), glial fibers acid protein(GFAP) immunohistochemical, immunofluorescence stain were used to observe the axonal regeneration, axon counting, glial scar formation and astrocytes neurite angle measurement. The statistical analysis was performed between 2 groups. Results： Compared with control group of 2w, BBB scores, MEP amplitude difference and axon count in experimental group showed no significant difference, while the MEP latency period of right hindlimb showed significant difference (P<0.05). At 6w,12w, BBB scores, MEP latency period difference and amplitude difference, axon count and astrocytes neurite angle measurement all showed significant difference (P<0.05) compared with control group. At 12 week, the HE dyeing histological result showed no difference between 2 groups, which both presented with syringomyelia and glial scar formation. As for NF200 immunohistochemical stain, more nerve fibers were noted in experimental group than control group; as for GFAP immunohistochemical stain, the density and IOD values showed no difference between two groups. Conclusions： Oscillating electrical field can promote motor function recovery in rats with spinal cord injury, the inner mechanism may be related with promoting axon regeneration, inducing the directional growth, promoting astrocytes linear array and providing axon outgrowth channels. |
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