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| CHEN Xuanwei,LIN Wenping,LIN Jianhua.The protection of lentivirus-mediated neuroglobin gene transfer in vivo on injured spinal cord in rabbits, an experimental study[J].Chinese Journal of Spine and Spinal Cord,2012,(3):272-277. |
| The protection of lentivirus-mediated neuroglobin gene transfer in vivo on injured spinal cord in rabbits, an experimental study |
| Received:July 31, 2011 Revised:October 31, 2011 |
| English Keywords:Spinal cord injury Neuroglobin Gene transfer Malondialdehyde Nitric oxide |
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| English Abstract: |
| 【Abstract】 Objectiveｓ: To investigate the possible protective mechanism of lentivirus-mediated neuroglobin gene transfer in vivo on injured spinal cord (SCI) in rabbits. Methodｓ: Ninety-six New Zealand white rabbits were randomly divided into four groups(n=24) according to intervention after SCI: no intervention(A); normal saline group(B); LV-GFP group (C); and LV-Ngb group(D).SCI was introduced by epidural balloon compression.24 hours after compression, T9 laminectomy was performed, then normal saline, LV-GFP or LV-Ngb was injected in situ respectively.Motor function of hind limbs after injection was evaluated by Basso-Beattie-Bresnahan(BBB) score, the expressions of green fluorescence in injured spinal cord were observed by fluorescence microscope, the expressions of Ngb mRNA and protein in injured spinal cord were detected by real-time PCR and Western blot, and the levels of malondialdehyde (MDA) and nitric oxide (NO) were also tested. Resultｓ： Significant functional improvement was observed in group D compared with group A, B and C at day 14 and 21.The expressions of green fluorescence in injured spinal cord were observed in group C and D.The Ngb expression in group D increased significantly compared with ｔｈｅ other 3 groups at day 7, 14 and 21.The MDA and NO levels in group D were lower than ｔｈｅ other 3 groups(P<0.05) at day 7, 14 and 21. Conclsionｓ： Lentivirus-mediated neuroglobin gene transfer in vivo may induce high expression of Ngb, which may be resulted from decreasing secondary spinal cord injury, thus promotｉｎｇ hind limbs motorfunction. |
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