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| YE Chaoqun,SUN Tiansheng,GAO Erjing.Migration and effect of cotransplantation of olfactory ensheathing cells（OECs） and Schwann cells（SCs）on axon regeneration in injured spinal cord[J].Chinese Journal of Spine and Spinal Cord,2010,20(2):138-145. |
| Migration and effect of cotransplantation of olfactory ensheathing cells（OECs） and Schwann cells（SCs）on axon regeneration in injured spinal cord |
| Received:May 12, 2009 Revised:December 07, 2009 |
| English Keywords:Spinal cord injury Olfactory ensheathing cells Schwanne cells Cell transplantation Migratation Axon regeneration |
| Fund:北京市委组织部优秀人才培养计划项目（20071D0502000266）；国家自然科学基金项目（30872604）；北京市虹天济神经科学研究院科研基金 |
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| English Abstract: |
| 【Abstract】 Objective：To investigate varied migration and effect of cotransplantation of olfactory ensheathing cells（OECs） and Schwann cells（SCs） on axon regeneration in injured spinal cord of rats.Method：Spinal cord contusion at T10 segment was induced in adult female SD rats（n=8，75±1d） by NYU impactor at the height of 25mm，and all animals were assigned as follows：（1）GFP+OECs cotransplantation 0.5mm cephalad and caudally to the injured site combined with injection of SCs labelled by hochest at injured site；（2）Mixed OECs and SCs（1：1）transplantation 0.5mm cephalad and caudally to injured site after 2 weeks（cell density：1×105/μl，depth：1.75mm，1.5mm，1.0mm，0.5mm，dosage：0.5μl cell suspension/per depth）.The rats were sacrificed at 1th and 8th week after transplantation respectively，and the T8-11 cord containing lesion and transplantation site were dissected，sectioned and stained immunochemically for fluorescent microscopy and then analsized for cell migration and axon regeneration.Result：After cotransplantation of OECs and SCs，SCs remained largely at the transplanted site，while OECs were capable of migrating from injection site to injured site longitudinally and laterally.Most of the OECs migrated to the injured site in gray matter and white matter，and some migrated through central canal or pia from the entry site. In transplantation of OECs and SCs to same site，OECs could migrate to the injury site，and SCs could migrate in a limited distance. After combined transplantation of OECs and SCs，NF+ and GAP-43+ axons were observed migrated parallelly to the OECs processes，transplantation with SCs contained a greater concentration of NF+ axons，but no GAP-43+ axons was evidenced.NF+ axons penetrated into the SCs grafts from all directions and twisted irregularly in the injured site.After transplantation of OECs and SCs into same site，numerous NF+ and GAP-43+ axons parallel to mixed cells processes were observed with the density of NF+ axons being low in injured site.Conclusion：Both mixed and combined transplantation of OECs and SCs has the capacity for cell migration and axon regeneration in contused spinal cord of rats，while the former is better than latter due to the good growth protocol. |
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