.Construction and identification of brain derived neurotrophic factor gene cloned into a T-vector in rat[J].Chinese Journal of Spine and Spinal Cord,2006,(4):.
Construction and identification of brain derived neurotrophic factor gene cloned into a T-vector in rat
Objective:To construct a T-vector cloned with a BDNF gene of a SD rat.Method:The total RNA of the brain of a SD rat were extracted,the first strand cDNA was synthesized using M-MLV reverse transcriptase and oligo-dT primers,and then BDNF cDNA was amplifie