| 王昊鹏,屠冠军,丛 琳.聚集蛋白聚糖基因串联重复多态性和肥胖交互作用与中国北方男性腰椎间盘突出症易感性的关系[J].中国脊柱脊髓杂志,2021,31(1):63-68. |
| 聚集蛋白聚糖基因串联重复多态性和肥胖交互作用与中国北方男性腰椎间盘突出症易感性的关系 |
| 中文关键词: 腰椎间盘突出症 聚集蛋白聚糖 基因串联重复多态性 肥胖 交互作用 |
| 中文摘要: |
| 【摘要】 目的：探讨中国北方汉族男性人群聚集蛋白聚糖（Aggrecan）基因串联重复多态性（variable number of tandem repeat，VNTR）与肥胖的交互作用对腰椎间盘突出症（LDH）发病的影响。方法：收集2004年1月～2015年12月在中国医科大学附属第一医院骨科住院且符合纳入及排除标准的299例男性志愿者的资料。病例组为101例临床诊断为LDH患者，对照组为198例无LDH症状的志愿者。对本研究知情同意，每人捐献一份血液样本。测量两组的身高、体重，计算体重指数（BMI）；采用PCR技术检测Aggrecan VNTR分布情况；应用Hwang′s模型分析Aggrecan VNTR与肥胖的交互作用对LDH发病的影响。结果：相较于既无VNTR重复次数≤25次等位基因危险因素也没有肥胖危险因素的个体，仅有VNTR重复次数≤25次等位基因危险因素而没有肥胖危险因素的个体的LDH发病率为1.027倍[P=0.938；95%CI：0.520～2.028]；仅有肥胖危险因素而没有VNTR重复次数≤25次等位基因危险因素的个体LDH发病率为1.050倍[P=0.885；95%CI：0.543～2.030]；既有VNTR重复次数≤25次等位基因危险因素也有肥胖危险因素的个体LDH发病率为4.521倍[P=0.000016；95%CI：2.277～8.978]。结论：中国北方男性LDH患者中，较短的Aggrecan VNTR串联重复序列片段（≤25）出现频率较高；Aggrecan基因VNTR与肥胖之间存在交互作用，影响LDH的发病。 |
The interaction between Aggrecan gene variable number of tandem repeat polymorphism and obesity in susceptibility of lumbar disc herniation in northern Chinese male |
| 英文关键词:Lumbar disc herniation Aggrecan Variable number of tandem repeat Obesity Interaction |
| 英文摘要: |
| 【Abstract】 Objectives: To study the interaction between Aggrecan gene variable number of tandem repeat (VNTR) polymorphism and obesity, and its effects on susceptibility of lumbar disc herniation(LDH) in Chinese Han male population lived in northern China. Methods: To collect the clinical data of 299 male volunteers who met the inclusion and exclusion criteria and were hospitalized in the Department of Orthopedics at the First Affiliated Hospital of China Medical University from January 2004 to December 2015. The case group included 101 patients clinically diagnosed with LDH and the control group included 198 volunteers without symptoms. Every subject gave informed consent to this study and donated a blood sample. Height and weight were measured and body mass index(BMI) was calculated. Aggrecan VNTR information was detected by PCR technique. The Hwang′s model was used to determine the interaction between aggrecan gene VNTR polymorphism and obesity, and its influence in susceptibility of LDH. Results: The data indicated that participants carrying allele ≤25 repeats who were non-obese showed a 1.027-fold increase in risk for LDH(P=0.938; 95%CI 0.520-2.028), and participants carrying two alleles >25 repeats who were obese showed a 1.050-fold increase risk(P=0.885; 95%CI 0.543-2.030), while participants carrying allele ≤25 repeats who were obese showed a 4.521-fold increase in risk for LDH(P=0.000016; 95%CI 2.277-8.978). Conclusions: In northern Chinese male LDH patients, shorter Aggrecan VNTR fragments(≤25 repeats) are more frequent, and there are interactions between Aggrecan gene VNTR polymorphism and obesity to affect LDH incidence. |
| 投稿时间：2020-06-29 修订日期：2020-10-07 |
| DOI： |
| 基金项目:国家自然科学基金面上项目（编号：81871803） |
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