| 马信龙,杨 召,孙晓雷,李秀兰,马剑雄,张 杨,郭 悦.周期性牵张应力对人退变髓核细胞增殖的影响[J].中国脊柱脊髓杂志,2012,(7):645-650. |
| 周期性牵张应力对人退变髓核细胞增殖的影响 |
| 中文关键词: 髓核细胞 周期性牵张应力 增殖 细胞周期 |
| 中文摘要: |
| 【摘要】 目的：观察周期性牵张应力对体外培养的人退变髓核细胞增殖的影响，为椎间盘退变的预防与治疗提供新的理论依据。方法：对体外培养的人退变髓核细胞利用甲苯胺蓝、番红O和Ⅱ型胶原免疫细胞化学染色进行鉴定，并将P3代细胞接种于弹性膜片上，待贴壁后移入ElectroForce 3200力学试验仪搭载的BioDynamic生物反应舱内对其施加0、5%、10%和15%的应变作用3h，受力完成后继续培养24h，用流式细胞仪测定细胞周期，计算S期百分数及增殖指数。结果：应力刺激后各组细胞贴壁及形态良好；应变作用3h后，0、5%、10%及15%应变组的S期百分数分别为1.86±1.22、2.07±0.09、7.56±1.40及7.55±3.25，增殖指数分别为3.47±2.52、7.88±2.45、16.05±1.51及14.7±0.61。5%应变组的增殖指数较0应变组明显增加（P＜0.05）；10%和15%应变组较0和5%应变组的S期百分数及增殖指数明显增加（P<0.05）；10%和15%应变组间比较差异无显著性（P＞0.05）。结论：在一定周期性应力范围内，髓核细胞的增殖能力增强。 |
Effects of cyclic distraction stress on proliferation of human nucleus pulposus cells |
| 英文关键词:Cyclic distraction stress Human nucleus pulposus Proliferation Cell cycle |
| 英文摘要: |
| 【Abstract】 Objectives: To investigate the effects of cyclic distraction stress on the proliferation of human nucleus pulposus cells(HNPCs). Methods: HNPCs were cultured in vitro and identified via the toluidine blue, safranin O and immunocytochemical stain of collagenⅡ. P3 HNPCs were inoculated on elasticity polymer. After HNPCs were attached, BioDynamic test instrument was used to exert different distraction strain(0, 5%, 10%, and 15%) on HNPCs for 3 hours; after that, HNPCs were cultured for 24 hours, and the flow cytometry was used to examine the cell cycle and calculate the S phase fraction and proliferative index. Results: The cell adhesion and growth were well under different strain for 3h; S phase fraction in 0, 5%, 10% and 15%strain groups was 1.86±1.22, 2.07±0.09, 7.56±1.40 and 7.55±3.25 respectively. Proliferative index in these groups was 3.47±2.52, 7.88±2.45, 16.05±1.51 and 14.7±0.61 respectively. Compared with 0 strain group, proliferative index in 5% strain group increased significantly(P<0.05). Compared with 0 and 5% strain group, S phase fraction and proliferative index in 10% and 15% strain groups increased significantly(P<0.05); but S phase fraction and proliferative index showed no significant difference between 10% and 15% strain groups(P＞0.05). Conclusions: The proliferation of HNPCs increases under some stress. |
| 投稿时间：2011-07-20 修订日期：2012-02-03 |
| DOI：10.3969/j.issn.1004-406X.2012.7.645.5 |
| 基金项目:国家自然科学基金青年基金项目（项目批准号：308011680）；天津市应用基础及前沿技术研究计划（项目编号：09JCZDJC19600）；天津市卫生局科研基金攻关项目（10KG111） |
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